Before I forget, these are some of the photos of the hydroxyapatite column shot today. The column is made from a PS pipette. But, I had to somehow switch it to a PP syringe cuz if I were to use a column of PS material, I would have to coat it with something to ensure there's no proteins sticking onto the wall of the PS column. PP is somehow highly recommended for use in proteome science cuz of its display of low adhesive force.
Hydroxyapatite is actually in white crystal form and is suspended in a 1mM phosphate buffer with a pH value of 6.8. When you pour the solution of the suspended hydroxyapatite crystals into the column, the crystals will settle to the bottom and form a gel bed.
I didn't get the column :(
ReplyDeletethe gel bed is the white colored bed. the column is the pipette itself. but i've switched to a 3mL syringe as the syringe is made of PP and is the most suitable for protein assays.
ReplyDeleteHi
ReplyDeleteIn the life sciences laboratory environment, there are few pieces of equipment which see more use than a multichannel pipette and along with it, pipette tips.
micropipette